山东医药2011，Vol.51Issue(52)：19-21,3.
以Cathelicidin序列为模板设计合成抗菌肽的抗菌特性研究
Biological activities of a synthetic antimicrobial peptide derived from the cathelicidin family
摘要
Abstract
Objective To study the effect of synthetic antimianrobial peptide derived from the Cathelicidin family on staphyoccocus aureus. Methods According to the method of NCCLS, the minimal inhibitory concentration ( MIC) and the minimal bactericidal concentration ( MBC) of the synthetic antimicrobial peptide against staphyoccocus aureus were deter mined. Peptide was incubated with a freshly prepared erythrocyte suspension from human blood (5% of normal hematocrit) and released hemoglobin which was determined photometrically at 540 nm. The peptide (80 μg/ml) was added to an over night culture of staphyoccocus aureus. This culture was placed at 37 ℃ and sampled every hour for 4 h and viable bacterial numbers were determined. Results The MIC was 16 μg/ml and the MBC was 128 μg/ml. The peptide exhibited hemolyt-ic activity below 10% at 100 μg/ml. After 4 h the vible bacteria in group A (antibacterial peptide) was significantly de creased compared with that in group C (control) (P<0.05). Conclusion The synthetic antimicrobial peptide possesses obvious anti-bacterial effect on staphyoccocus aureus and displays less hemolytic activity.关键词
抗菌肽/金黄色葡萄球菌/抑菌作用Key words
antimicrobial peptide/ staphyloccocus aureus/ antibacterial activity分类
生物科学引用本文复制引用
陈曼,胡启飞,陈登榜,吴民泸,代娟,赖翼,段佳慧..以Cathelicidin序列为模板设计合成抗菌肽的抗菌特性研究[J].山东医药,2011,51(52):19-21,3.基金项目
四川省教育厅科研项目(09ZC078). （09ZC078）
