山东医药2011，Vol.51Issue(52)：34-36,3.
参附注射液对人慢性髓性白血病细胞来源的树突状细胞诱导生成的影响
Promotion of shenfu Injection on inducing chronic myeloid leukemia cell into dendritic cells
任莉莉 1问明 1杨华 1臧爱民 1贾友超 1魏影非2
作者信息
- 1. 河北大学附属医院,河北保定071000
- 2. 河北医科大学第三医院
- 折叠
摘要
Abstract
Objective To investigate the effects of shenfu injection (SFI) on the induction of chronic myeloid leuke mia cell line K-562 cells into dendritic cells (DCs). Method In vitro, K-562 cells were cultured with different concen trations SFI, granulocyte-macrophage colony-stimulating factor ( GM-CSF)/interleukin-4 (IL-4) and GM-CSF/IL-4 com bined with different concentrations of SFI, respectively. Results In vitro, DCs could not be derived from K-562 cells cul tured with different concentrations of SFI. While DCs derived from K-562 cells co-cultured with GM-CSF/IL-4 could ex press phenotype of antigen-presenting cells and have the similar immune function with normal DCs, but the surface markers and the function were unmature. However, different concentration of SFI plus GM-CSF/IL-4 could not only induce matura tion of K-562 DCs but inhibit propagation of cells. Cells cultured with middle concentration SFI (75 mg/ml) plus GM-CSF/IL-4 expressed higher levels of phenotypes, elicited significantly stronger abilities of stimulating T lymphocyte proliferation, and secreted larger amounts of IL-12. Conclusions In vitro, appropriate concentrations of SFI plus GM-CSF/IL-4 can induce differentiation and maturation of K-562 DCs, change the expression of their surface markers and enchance their immune stimulatory function. The best concentration of SFI is 75 mg/ml which plus GM-CSF/IL-4 can induce K-562 cells into DCs.关键词
参附注射液/白血病/树突状细胞/K-562细胞Key words
shenfu injection/ leukemia/ dendritic cells/K-562 cell分类
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任莉莉,问明,杨华,臧爱民,贾友超,魏影非..参附注射液对人慢性髓性白血病细胞来源的树突状细胞诱导生成的影响[J].山东医药,2011,51(52):34-36,3.
