山东医药2012，Vol.52Issue(2)：4-6,3.
Notch1信号通路在人脑胶质瘤U87细胞增殖、凋亡中的作用及机制探讨
Effect and mechanism of Notch1 signaling pathway on proliferation and apoptosis in human glioma U87 cells
摘要
Abstract
Objective To investigate the effect of Notchl signaling pathway on the proliferation and apoptosis of human glioma U87 cells and explore the mechanism. Methods Human human glioma U87 cells were randomly divided into 3 group, and cocultured with Notch activator rhNF-kB、Notch inhibitor DAFT and PBS. MTT assay was used to detect the effect on U87 cells proliferation, flow cytometry technique was also employed to detect the effects on apoptosis. RT-PCR and Western-blot were employed respectively to evaluate the mRNA and protein expressions of Notchl、Hesl and Bcl-2 in U87 cells. Results A value of U87 cells cultured for 24,48,72,96 h were 0. 185 ± 0. 007,0. 398 ±0. 012,0. 735 ± 0.015,1.083 ±0.031 in group A;0. 102 ±0.003,0. 130 ±0.004,0. 161 ±0.006,0. 194 ±0.003 in group B; 0. 167 ± 0.005,0. 265 ± 0. 008,0.496 ±0.011,0.737 ±0.016 in group C. A value in group A , B had significant difference cop-ared with group C(all P<0.05). Cell apoptosis ratio in group A,B,C wereO.96% ±0. 17% ,26.51% ±3.74% ,8.76% ± 1.40% . Cell apoptosis ratio in group A , B had significant difference copared with group C ( all P < 0. 05 ). Notchl, Hesl , Bcl-2 mRNA and protein in group A, B had significant difference copared with group C(all P <0. 05). Conclusions Notchl sigaling pathway plays an important regulating role in the proliferation and apoptosis of human glioma U87 cells, and the probable mechanisms maybe that it can control the expressions of the target gene Hesl and anti-apoptosis protein Bcl-2.关键词
Notch1信号通路/Hes1基因/Bcl-2基因/细胞凋亡/细胞增殖/胶质瘤/人脑胶质瘤细胞Key words
Notchl signaling pathway/ Hesl gene/ Bcl-2 gene/ apoptosis/ proliferation/ glioma/ human glioma cells分类
医药卫生引用本文复制引用
刘锋,吕世刚,祝新根,周椿昊,李建斌,徐文华..Notch1信号通路在人脑胶质瘤U87细胞增殖、凋亡中的作用及机制探讨[J].山东医药,2012,52(2):4-6,3.基金项目
江西省科技厅重点支持项目(2010BSA12300) （2010BSA12300）
江西省教育厅科学技术研究项目(GJJ01863) （GJJ01863）
江西省研究生创新专项资金项目(YC10A027). （YC10A027）
