山东医药2012，Vol.52Issue(3)：1-4,4.
BAG-1基因沉默对非小细胞肺癌放射敏感性的影响及机制
Effect and mechanism of BAG-1 gene silencing on radio-sensitivity in non-small cell lung cancer
摘要
Abstract
Objective To explore the relationship between BAG-1 and radiation sensitivity of non-small cell lung cancer so to provide for theoretical basis for individual therapy of non-small cell lung cancer. Methods Radiation sensi-tivity experiment was used in A549 cells, H460 cells, NCL-H520 cells and HEK293 cells, expression of BAG-1 protein was observed in different cell lines through Western blot, then the A549 cells which relatively resistant to radiation and ex-pressed BAG-1 protein highest were chosen, and RNA interference vector pGCsi-BAG-1 was constructed, silencing BAG-1 gene cell lines shRNA-2 were selected, growth of cells were observed using microplate reader, apoptosis was detected through flow cytometry, radiosensitivity was studied using radio sensitivity test. Results Silencing BAG-1 gene shRNA-2 inhibited cell growth, which was closely related to growth time, shRNA-SC cells had no significant effect on cell growth; 24 h after the radiation of 6 Gy X-ray, apoptosis rate of shRNA-2 transfected cells was ( 49. 6 ± 4. 23 ) % , the control cells was (15. 3 ±2. 11) % , the shRNA-SC cells was( 14. 8 ± 3. 55) % , apoptosis rate of shRNA-2 transfected cells increased 3. 2 fold compared with control cells(P <0. 01 ) , while which of shRNA-SC cells had no significant difference compared with control cells; no difference of radiosensitivity was found between the parent A549 cells and shRNA-SC cells, but the radio-sensitivity of shRNA-2 transfected cells was significantly higher than that of the former cells (P > 0. 05). Conclusions BAG-1 gene silencing can enhance radiosensitivity of non-small cell lung cancer, its mechanism maybe that BAG-1 gene silencing can inhibit cell growth, increase apoptosis, and change intracellular distribution of BAG-1, make the cells more sensitive to radiation.关键词
BAG-1基因/癌,非小细胞肺/放射敏感性Key words
BAG-1 gene/ cancer, non-small cell lung/ radiosensitivity分类
医药卫生引用本文复制引用
杨青山,刘媛媛,姜立朋..BAG-1基因沉默对非小细胞肺癌放射敏感性的影响及机制[J].山东医药,2012,52(3):1-4,4.基金项目
辽宁省科学技术厅博士启动基金资助项目(20111115) （20111115）
辽宁医学院院内青年基金资助项目(y2010z016). （y2010z016）
