山东医药2012，Vol.52Issue(3)：5-8,4.
百日咳鲍特菌BP283基因的实时荧光定量PCR检测方法的建立
Development of real time fluorescent quantitative PCR for detection of Bordetella pertussis BP283 gene
摘要
Abstract
Objective To evaluate the effective method for detection of Bordetella pertussis BP283 gene. Methods The primers and Taqman probe were designed according to the sequence BP283 of Bordetella pertussis, and the standards were constituted; then the real time fluorescent quantitative PCR(FQ-PCR) reaction system was optimized and evaluated. Results The recombinant vector (pCR2. 1-BP283) was cloned successfully; the FQ-PCR was well established; the corre-lation coefficient of standard curve was 0.998, the minimum detection limit concentration was 10 copies/μL , no specific amplification curve for the other common clinical pathogen in respiratory tract was found, both intra and inter assay coeffi-cients of variation were lower than 4% . Conclusion FQ-PCR can detect Bordetella pertussis BP283 gene successfully with good sensitivity, specificity and reproducibility.关键词
百日咳鲍特菌/实时荧光定量/BP283基因Key words
Bordetella pertussis/ real time fluorescent quantitative/ BP283 gene分类
医药卫生引用本文复制引用
罗炜,王慧,刘利东,任筱兰,赖克方..百日咳鲍特菌BP283基因的实时荧光定量PCR检测方法的建立[J].山东医药,2012,52(3):5-8,4.基金项目
广东省科技计划基金资助项目(2010B031600145). （2010B031600145）
