山东医药2012，Vol.52Issue(3)：15-18,4.
下呼吸道感染患者铜绿假单胞菌分离株氨基糖苷类耐药相关基因分析
Analysis of genes related to aminoglycoside-resistance among Pseudomonas aeruginosa strains isolated from patients with lower respiratory tract infection
摘要
Abstract
Objective To explore the resistant mechanism to aminoglycosides among Pseudomonas aeruginosa ( PAE) strains isolated from patients with lower respiratory tract infection. Methods Total of 52 aminoglycosides-resistant PAE strains were isolated from sputum of patients with lower respiratory tract infection, 6 kinds of aminoglycosides modification enzymes ( AMEs) genes among all the isolates and 6 kinds of 16S rRNA methylase genes among the isolates that pan-resist-ant to aminoglycosides sequenced. Results Among 52 isolates of PAE, 4 kinds of AMEs genes [aac(3)-Ⅱ , aac(6')- Ⅰ b, aac(6')-Ⅱ andant(2")-I ] were found. The total positive rates of AMEs genes was 92. 3% . Among the isolates that pan-resistant to aminoglycosides, 1 kind of 16S rRNA methylase gene (rmtB) was found. The positive rate of rmtB in 16 i-solates that high level pan-resistant to aminoglycosides was 81.3%. Conclusion The positive rate of AMEs genes is very high among aminoglycosides-resistant PAE strains isolated from patients with lower respiratory tract infection. The AMEs genes related to aminoglycosides resistance include aac(3)-Ⅱ , aac(6')- Ⅰ b, aac(6')-Ⅱ and ant(2")- Ⅰ . The high level pan-resistance to aminoglycosides is mainly related to 16S rRNA methylase gene rmtB.关键词
下呼吸道感染/铜绿假单胞菌/氨基糖苷类修饰酶/16S rRNA甲基化酶/基因Key words
lower respiratory tract infection/ Pseudomonas aeruginosa/ aminoglycosides modification enzymes/ 16S rRNA methylases/ gene分类
医药卫生引用本文复制引用
毕玲,葛庚芝,赖雁平,于树云,司进,李彬,张贵生..下呼吸道感染患者铜绿假单胞菌分离株氨基糖苷类耐药相关基因分析[J].山东医药,2012,52(3):15-18,4.基金项目
天津医科大学科学基金资助项目(2009ky39). （2009ky39）
