山东医药2012，Vol.52Issue(3)：25-27,3.
苦参碱对人肺腺癌A549细胞增殖及HIF-1α、VEGF表达的影响
Effect of matrine on proliferation and HIF-1α,VEGF expression of A549 cells
摘要
Abstract
Objective To explore the effect and mechanism of matrine on the proliferation of A549 cells. Methods A549 cell line in logarithmic growth cultured in vitro was treated by matrine with the concentration of 0, 5 , 50, 100, 250, 500 μg/mL, respectively, and then cellular proliferation was evaluated by MTT method. Hypoxia-inducible factor-1α(HIF-1α )and vascular endothelial growth factor (VEGF) mRNA expression in A549 cells were detected by RT-PCR analysis. Results Matrine inhibited the proliferation of A549 cells in a dose and time-dependent manner(P <0. 05) ; inhibited the expression of HIF-1α, VEGF mRNA in a dose and time-dependent(P <0. 05) ; there was a positive correlation between the expression of HIF-1α mRNA and VEGF mRNA(r =0.994,P <0. 01). Conclusion Matrine can inhibit the proliferation of A549 cell line, the mechanism may down-regulate the expression of HIF-lα and VEGF, so to inhibit the vascular growth of lung cancer tissue.关键词
苦参碱/肺肿瘤/缺氧诱导因子-1α/血管内皮生长因子Key words
matrine/ lung tumor/ hypoxia-inducible factor-1α/ vascular endothelial growth factor分类
医药卫生引用本文复制引用
周娟,倪松石,贲素琴..苦参碱对人肺腺癌A549细胞增殖及HIF-1α、VEGF表达的影响[J].山东医药,2012,52(3):25-27,3.基金项目
南通市社会发展科技计划资助项目(S2009061). （S2009061）
