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苦参内生真菌的分离鉴定及抑菌活性的初步研究

何璐 纪明山 刘娜 徐洪波 齐补坤

上海农业学报2011,Vol.27Issue(4):83-86,4.
上海农业学报2011,Vol.27Issue(4):83-86,4.

苦参内生真菌的分离鉴定及抑菌活性的初步研究

Isolation and identification of an entophytic fungus from Sophora flavescens and its microbiostatic activity

何璐 1纪明山 2刘娜 1徐洪波 3齐补坤2

作者信息

  • 1. 沈阳农业大学植物保护学院,沈阳116021
  • 2. 辽宁科技大学化工学院,鞍山114051
  • 3. 上海市农业科学院园艺研究所,上海市设施园艺技术重点实验室,上海201403
  • 折叠

摘要

Abstract

An entophytic fungus was separated from the fresh seed of Sophora flavescens by using tissue isolation and then identified in terms of morphology and 16S rDNA molecular biology to confirm the strain name. Also its effects on common agricultural pathogenic fungi and bacteria were determined. The results showed that the colony of the entophytic fungus separated and purified from the fresh seed is light yellow and has a short-fluff form and a few of radial grooves .its vegetative mycelia are tree-root-shaped,the conidiophore is relatively long with short-rod-like apex,and the conidium is oval.The fungus was identified as Aspergillus terreu. Microbiostatic tests indicated that the strain has a significant inhibiting effect on several agricultural pathogenic fungi and bacteria, and especially on Enterobacter aerogenes and Fusarium oxysporum f. Sp. Cucumerinum.

关键词

苦参/内生真菌/土曲霉/分离纯化/抑菌活性

Key words

Sophora flavescens/Endophytic fungi/Aspergillus terreus/Separation and purification/Microbiostatic activity

分类

农业科技

引用本文复制引用

何璐,纪明山,刘娜,徐洪波,齐补坤..苦参内生真菌的分离鉴定及抑菌活性的初步研究[J].上海农业学报,2011,27(4):83-86,4.

基金项目

鞍山市科技局项目(000243)资助 (000243)

上海农业学报

OA北大核心CSCDCSTPCD

1000-3924

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