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中国沿海管角螺4个自然群体遗传多样性的RAPD分析

罗福广 苏翔驹 区小玲 李斌 潘英 郑惠芳 覃志彪

上海海洋大学学报2011,Vol.20Issue(5):655-660,6.
上海海洋大学学报2011,Vol.20Issue(5):655-660,6.

中国沿海管角螺4个自然群体遗传多样性的RAPD分析

RAPD analysis on Hemifusus tuba(Gmelin) of four different natural populations in China

罗福广 1苏翔驹 1区小玲 1李斌 1潘英 1郑惠芳 1覃志彪1

作者信息

  • 1. 广西大学动物科学技术学院,广西南宁530004
  • 折叠

摘要

Abstract

Nineteen RAPD primers amplified a total of 182 scorable markers in 200 samples from the four different natural populations of H.tuba,thus a total of 19 983 bands which ranged in size from 250 to 2 500 bp were generated showing homogeneous RAPD patterns,and number of scorable bands for each primer varied from 4 to 17 with an average of 5.28 bands per RAPD primer.81.87% of the total genetic diversity was found among the four populations which contained approximately 149 polymorphic loci.66.48%,47.25%,44.51%,36.81% of the total genetic diversity were attributable to genetic differences within Beihai,Zhanjiang,Wenzhou,Lianyungang populations.Estimates of Nei’s Gene Diversity and the Shannon’s Diversity Index provided similar results of diversity,from high to low,that is,Beihai,Zhanjiang,Wenzhou,Lianyungang populations.The cluster analysis of the four populations was based on Genetic Distance,which was measured between 0.054 2-0.137 4.The results showed the populations of Beihai and Zhanjiang first assembled one branch,Wenzhou and Lianyungang then assembled another,and the two branches assembled finally.

关键词

管角螺/遗传多样性/遗传距离/RAPD

Key words

Hemifusus tuba (Gmelin)/genetic diversity/genetic distance/RAPD

分类

农业科技

引用本文复制引用

罗福广,苏翔驹,区小玲,李斌,潘英,郑惠芳,覃志彪..中国沿海管角螺4个自然群体遗传多样性的RAPD分析[J].上海海洋大学学报,2011,20(5):655-660,6.

基金项目

国家自然科学基金 ()

广西科学研究与技术开发计划项目 ()

上海海洋大学学报

OA北大核心CSCDCSTPCD

1674-5566

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