上海海洋大学学报2012，Vol.21Issue(1)：21-27,7.
草鱼JAK2基因片段序列的克隆及其组织表达分析
Cloning and tissue expression of JAK2 gene in grass carp（Ctenopharyngodon idella）
摘要
Abstract
The JAK2（Janus kinase 2） gene fragment sequence was cloned from liver of grass carp（Ctenopharyngodon idella） by RT-PCR.The obtained cDNA fragment sequence was 671 bp,which encoded 223 amino acids.The amino acid sequence comparison results showed that the JAK2 amino acid of grass carp shared 70%-91% identity with other species.Phylogenetic tree of JAK2 generated by Neighbor Joining method suggested that all teleosts cluster together,and the grass carp JAK2 is clustered closely with that of zebrafish,mandarin fish and globefish.Then the expressions of different tissues including liver,muscle,brain,heart,spleen and mesentery fat tissue were analyzed by real-time fluorescence quantitative PCR.The results showed that the JAK2 gene of grass carp expressed in all tissues and highest in liver（P0.05）,lower in muscle,brain,spleen and fat tissue,lowest in heart（P0.05）.This study will be helpful for further understanding the structure and function of JAK2 in C.idella.关键词
草鱼/JAK2/基因克隆/实时荧光定量PCR/组织表达Key words
Ctenopharyngodon idella/JAK2/gene cloning/real-time PCR/tissue expression分类
农业科技引用本文复制引用
吴小凤,李小勤,冷向军,关磊,郭婷..草鱼JAK2基因片段序列的克隆及其组织表达分析[J].上海海洋大学学报,2012,21(1):21-27,7.基金项目
上海市教育委员会发展基金 （）
上海海洋大学“教育部水产种质资源发掘与利用省部共建重点实验室”及“农业部水产种质资源与利用重点开放实验室”开放课题基金 （）
中国南海水产研究所种质资源开放课题 （）
上海市重点学科建设项目 （）
