实验动物与比较医学2012，Vol.32Issue(1)：47-50,59,5.DOI:10.3969/j.issn.1674-5817.2012.01.012
葡聚糖硫酸钠和偶氮氧甲烷建立小鼠溃疡性结肠炎癌变模型
A Novel Mouse Model for Ulcerative Colitis-associated Carcinogenesis Induced by Azoxymethane and Dextran Sulfate Sodium
摘要
Abstract
Objective To develop an ulcerative colitis-associated carcinogenesis model in mice. Methods Female B ALB/c mice were randomly divided into 4 groups: the control group, the azoxymethane (AOM) group, the dextran sulfate sodium (DSS) group and the AOM + DSS group. AOM was intraperi-toneally injected. DSS was fed for 1 week then with ordinary water for 2 weeks, such 3 weeks formed a cycle and 9 weeks in all. The body weight was detected weekly and the mental status, stool character, hairs glossy and mortality were daily observed and recorded. Levels of TNF-a was tested by ELISA. Inflammation and pathology were observed by HE staining. Results ① After drinking DSS,the mice showed diarrhea, bloody stool, weight loss, depressed and hairs messy. Post-oral tumor were seen anal prolapse. Non-DSS groups had no more performance. AOM + DSS group appeared one death. The body weight was significantly decreased at 4,7,8,9 week after treatment compared with the control group (P <0.05). ② The TNF-a was significantly increased in DSS groups (P<0.05). ③ HE staining: DSS groups showed infiltration of inflammatory cells and (or) the formation of lymphoid follicles. The tumors were induced in all of the AOM + DSS mice (9/9), which were most located in distal colon and the pathology were showed high atypical hyperplasia and (or) carcinoma in situ. Dysplasia or cancer was not observed in the other three groups. Conclusion Ulcerative colitis-associated carcinogenesis model in mice can prepared with a single intraperitoneal injection of azoxymethane prior to three repetitive oral administrations of dextran sulfate sodium. This method have the advantages with shorter time, high formation of tumor and stability of histological type.关键词
溃疡性结肠炎/结直肠肿瘤/偶氮氧甲烷/葡聚糖硫酸钠/BALB/c小鼠Key words
Ulcerative colitis/ Colorectal cancer/ Azoxymethane/ Dextran sulfate sodium/ BALB/c mice分类
医药卫生引用本文复制引用
郑海明,赵航,郑萍..葡聚糖硫酸钠和偶氮氧甲烷建立小鼠溃疡性结肠炎癌变模型[J].实验动物与比较医学,2012,32(1):47-50,59,5.基金项目
上海市科学技术委员会科研计划项目 (08140902800) （08140902800）
