生命科学研究2011，Vol.15Issue(6)：512-518,7.
人诱导性多潜能干细胞(iPS细胞)系的建立
Generation of A Human Induced Pluripotent Stem (iPS) Cells Line
摘要
Abstract
To master the technology of reprogramming human somatic cells to iPS cells and set up a technical platform to reprogram human cancer cells into iPS cells. Under human embryonic stem cell (hES cell) culture conditions, human skin fibroblasts (CCD-1079SK) cells infected by lentivirus mixture harboring Oct4, Sox2, c-Myc and Klf4 genes were induced into hES cell-like colonies, following characterization were done by comparatively analyzing hES cells markers: colony morphology, alkaline phosphatase (AP) activity, the expression profile of ES cell-marker genes, karyotype and embryoid body (EB)-mediated in vitro differentiation of CCD-1079SK cell-derived hES cell-like colonies. The results showed that human iPS cell line (iPSC-1) was generated from CCD-1079SK cells by introducing four genes, Oct4, Sox2, c-Myc and Klf4, under hES cell culture conditions. iPSC was similar to hES cells in morphology, proliferation, hES cell-specific surfaceanti-gens and gene expression. Additionally, iPSC could be cultured in suspension to form embryoid bodies (Ebs) and differentiate into cell types of the three germ layers in vitro, in addition, human iPS cell line is successfully established, which will lay a solid foundation for performing cancer cell reprogramming by using iPS cell technology.关键词
人诱导性多潜能干细胞/肿瘤细胞重编程/肿瘤治疗Key words
human induced pluripotent stem (iPS) cells/cancer cell reprogramming/cancer therapy分类
医药卫生引用本文复制引用
姚志芳,史俊文,贾俊双,申红芬,林晓琳,肖高芳,张晟,肖东,姚开泰..人诱导性多潜能干细胞(iPS细胞)系的建立[J].生命科学研究,2011,15(6):512-518,7.基金项目
国家自然科学基金委员会-广东省联合基金重点项目(u0732006) （u0732006）
广东省自然科学基金资助项目(9151063101000015) （9151063101000015）
广东省科技计划项目(2009B060300008) （2009B060300008）
南方医科大学优秀中青年科技人才库科研资助项目 （）
