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基于生长时间光谱法的大肠菌群单管定量检测技术研究

蔡尽忠 庄峙厦 赵丽 汤新华 鄢庆枇

生命科学仪器2011,Vol.9Issue(5):46-48,3.
生命科学仪器2011,Vol.9Issue(5):46-48,3.

基于生长时间光谱法的大肠菌群单管定量检测技术研究

Establishment of Single Tube Quantitative Detection Technology for Coliforms Assay Based on Time of Growth Spectrophotometer Method

蔡尽忠 1庄峙厦 1赵丽 2汤新华 1鄢庆枇3

作者信息

  • 1. 厦门华厦职业学院食品药品安全检测实验室,福建厦门361024
  • 2. 厦门大学化工学院,福建厦门361005
  • 3. 厦门斯坦道生物公司,福建厦门361006
  • 折叠

摘要

Abstract

By studying the initial inoculation concentration of coliforms and the time required to reach 0.2 absorbance,and establishment of single tube quantitative detection technology for coliforms assay based on time of growthspectrophotometer method.By comparison with the multi-tube fermentation method of quantitative detection of single-tube technology that the relative standard deviation less than the conventional multi-tube fermentation method,and the reaction time is short,when the initial bacterial concentration was 101.3cfu/mL,the reaction time was only 9h,can serve as a method of rapid detection of coliforms,has a good prospect.

关键词

大肠菌群/ONPG/生长时间光谱法/单管定量检测

Key words

coliforms/ONPG/time of growth spectrophotometer method/single-tube quantitative detection

分类

轻工纺织

引用本文复制引用

蔡尽忠,庄峙厦,赵丽,汤新华,鄢庆枇..基于生长时间光谱法的大肠菌群单管定量检测技术研究[J].生命科学仪器,2011,9(5):46-48,3.

基金项目

厦门市科技计划创新项目 ()

生命科学仪器

1671-7929

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