色谱2011，Vol.29Issue(12)：1210-1215,6.DOI:10.3724/SP.J.1123.2011.01210
高效液相色谱法考察60Co-γ射线照射天麻其化学成分的稳定性
Stability evaluation of chemical compositions of Rhizoma gastrodiae with 60Co-γirradiation by high performance liquid chromatography
摘要
Abstract
The main chromatographic fingerprint peaks of Rhizoma gastrodiae were established for evaluating the stability of chemical compositions of Rhizoma gastrodiae with 60Co-γ irradiation to control the dosage of 60Co-γ irradiation sterilization by high performance liquid chroma-tography (HPLC). Eight Rhizoma gastrodiae samples were analyzed. The chromatographic fingerprints of Rhizoma gastrodiae were constructed with 14 common fingerprint peaks, and its 10 main peaks were identified using the similarity evaluation system of chromatographic fingerprints of traditional Chinese medicine (version 2004 A) recommended by State Food and Drug Administration of China. The relative standard deviations of the peak areas of the 10 main peaks were used to evaluate the stability of corresponding chemical compositions of Rhizoma gastrodiae with 60Co-γ irradiation. The results showed that the compositions of 3 of the 10 main peaks were influenced and a good relationship was obtained between the influence and 60Co-γ irradiation doses for the eight Rhizoma gastrodiae samples. Some chemical components of Rhizoma gastrodiae weren't steady after 60Co-γ irradiation, so a low dosage should be selected for 60Co-γ irradiation sterilization.关键词
高效液相色谱/60Co-γ照射/主要指纹峰/稳定性/天麻Key words
high performance liquid chromatography (HPLC) / 60Co-γ irradiation/ main fingerprint peak/ stability/ Rhizoma gastrodiae分类
化学化工引用本文复制引用
杨孝容,黄敏,刘素君,宋九华..高效液相色谱法考察60Co-γ射线照射天麻其化学成分的稳定性[J].色谱,2011,29(12):1210-1215,6.基金项目
四川省科技支撑计划项目(2009sz127) （2009sz127）
