色谱2011，Vol.29Issue(12)：1236-1239,4.DOI:10.3724/SP.J.1123.2011.01236
凝胶渗透色谱净化-高效液相色谱法测定油脂食品中的邻苯二甲酸酯类增塑剂
Determination of phthalate plasticizers in foods by high performance liquid chromatography with gel permeation chromatographic clean-up
摘要
Abstract
A method of gel permeation chromatography-high performance liquid chromatogra-phy (GPC-HPLC) was established for the simultaneous determination of 5 main phthaiate plas-ticizers in foods (edible oil, instant noodles, fried pastries, Saqima, etc. ). The samples were extracted with petroleum ether in an ultrasonator, purified by a GPC column, and analyzed by HPLC. The chromatographic separation was achieved on a Labtech-C18 column (250 mm ×4. 6 mm, 5μm} using acetonitrile and water mixture as the mobile phases in a gradient elution mode. The developed method exhibited a linear correlation coefficient of more than 0. 997 and the detection limits of 3. 25 - 13. 4 μg/L. The spike recoveries were between 70. 4% and 113.6% with the relative standard deviations (RSDs, n=3) of 0.3%-5.8% at the spiked level of 50 mg/L. This method is simple, rapid and practical, and can be used for the simultaneous determination of PAEs in grease food samples.关键词
凝胶渗透色谱/高效液相色谱/邻苯二甲酸酯/增塑剂/油脂食品Key words
gel permeation chromatography (GPC) / high performance liquid chromatography (HPLC)/ phthalates/ plasticizers/ grease food分类
化学化工引用本文复制引用
张春雨,王辉,张晓辉,马忠强,邓宛梅,胡克,丁明玉..凝胶渗透色谱净化-高效液相色谱法测定油脂食品中的邻苯二甲酸酯类增塑剂[J].色谱,2011,29(12):1236-1239,4.基金项目
国家自然科学基金项目(No.21075074) （No.21075074）
