生态学杂志2012，Vol.31Issue(1)：100-105,6.
邻苯二甲酸二乙基己酯对翡翠贻贝(Perna viridis)生化指标的影响
Effects of di-(2-ethylhexyl)phthalate on the biochemical indices of green mussel(Perna viridis)
摘要
Abstract
A laboratory experiment was carried out to study the variations of superoxide dismutase (SOD) and catalase (CAT) activities and malondialdehyde (MDA) content in the visceral mass and mantle of green mussel (Perna viridis) exposed to different concentrations (0, 0.38, 1.92, 9. 60, and 48. 00 mg ? L-1) of di-(2-ethylhexyl) phthalate (DEHP) for a long term, and the recovery of these indices after the stress released. During the exposure period, the SOD activity in the visceral mass had an initial significant increase (P<0. 05) and followed by a gradual de crease , while the CAT activity was inhibited first but induced then, recovering to the level of the control after 15 days of exposure. The MDA content in the visceral mass increased significantly with increasing exposure time (P<0. 05). In the early period of exposure, the SOD activity in the mantle of the P. Viridis groups exposed to lower concentrations DEHP was inhibited but that of the groups exposed to higher concentrations DEHP was induced (P<0. 05) , while after 4 days of exposure, the SOD activity of all the groups recovered to the normal level. The MDA content in the mantle of all the groups increased significantly (P<0. 05). During the releasing period, the SOD and CAT activities in the visceral mass of P. Viridis exposed to 0. 38 mg ? L-1 of DEHP recovered gradually to the normal level, but the MDA content increased. After 7 days of relea sing , the SOD activity in the mantle of P. Viridis exposed to all test concentrations (except 48. 00 mg ? L-1) of DEHP recovered to the normal level, while the MDA content maintained at a stable level. The above results indicated that DEHP had no obvious effects on the enzymes of antioxi-dant defense systems in the visceral mass and mantle of P. Viridis but could induce the lipid per-oxidation damage in the 2 tissues, and this damage could not recover in a short term.关键词
邻苯二甲酸二乙基己酯/翡翠贻贝/丙二醛/毒性效应Key words
di-(2-ethylhexyl) phthalate (DEHP)/Perna viridis/malondialdehyde (MDA)/toxic effect.分类
农业科技引用本文复制引用
蔡文贵,秦洁芳,贾晓平,陈海刚,陈炜婷,马胜伟,张喆,倪朝辉..邻苯二甲酸二乙基己酯对翡翠贻贝(Perna viridis)生化指标的影响[J].生态学杂志,2012,31(1):100-105,6.基金项目
中国水产科学研究院淡水生态与健康养殖重点开放实验室开放课题(2010FEA03006)、广东省科技计划项目(2009 8030600001)、科技部科研院所社会公益研究专项(2005 DIB3J021)、中央级公益性科研院所基本科研业务费专项资金项目(2009YD01)和中央级公益性科研院所基本科研业务费专项资金项目(2010YD04)资助. （2010FEA03006）
