生物骨科材料与临床研究2012，Vol.9Issue(1)：21-25,5.DOI:10.3969/j.issn.1672-5972.2012.01.006
多孔纳米羟基磷灰石/壳聚糖支架材料复合成骨细胞的异位成骨研究
Ectopic osteogenesis of osteoblasts composite Porous-Nano-hydroxyapatite/chitosan
陆钰 1王鑫 2甘朝兵 2赵玫 2储岚岚 1康林 2吴海珍1
作者信息
- 1. 安徽医科大学解放军临床学院口腔科,安徽合肥230031
- 2. 中国人民解放军第一0五医院口腔科,安徽合肥230031
- 折叠
摘要
Abstract
Objective To evaluate the ability of ectopic osteogenesis of composites osteoblasts with Porous-Nano-hydro-xyapatite/chitosanC nHA/CS)in rat's muscle pouch. Methods Pro-nHA/CS was prepared through co-precipitation and particle-leaching method. The rat osteoblasts were isolated and cultured in vitro. Then we use the osteoblasts and pro-nHA/ CS to construct tissue engineered bone. Then the tissue engineered bone and the pro-nHA/CS were implanted in to rats' thigh muscle pouch. Specimens were harvested at week 2,4,6 and 8 and analyzed by hemotoxylin and eosin staining. The percentages of new bone were determinate by JEDA-801D software. SPSS 13.0 software package was used for one-way ANOVA. Results In each observation period, osteoblasts with porous nHA/CS group new bone density are more than nHA/CS group. Conclusion The composite of osteoblasts with pro- nHA/CS scaffold material has the ability of ectopic osteogenesis.关键词
多孔纳米羟基磷灰石/壳聚糖/成骨细胞/异位成骨Key words
Pro-nHA/CS/ Osteoblasts/ Ectopic osteogenesis分类
医药卫生引用本文复制引用
陆钰,王鑫,甘朝兵,赵玫,储岚岚,康林,吴海珍..多孔纳米羟基磷灰石/壳聚糖支架材料复合成骨细胞的异位成骨研究[J].生物骨科材料与临床研究,2012,9(1):21-25,5.
