生物加工过程2011，Vol.9Issue(6)：11-14,4.DOI:10.3969/j.issn.1672-3678.2011.06.003
氮源对重组大肠杆菌发酵产L-精氨酸的影响
Effect of nitrogen sources on L-arginine production by recombinant E.coil
摘要
Abstract
The effect of nitrogen sources on L-arginine production by recombinant E. Coli was studied. The effects of the varieties of organic nitrogen sources, peptone concentration, and (NH4)2SO4 concentration on L-arginine production were investigated. When the concentration of peptone and (NH4)2 SO4 were 10 g/L and 15 g/L, respectively, 9.4 g/L of L-arginine was obtained in shake flask culture. In batch fermentation with a 5-L fermentor, 18. 8 g/L of L-arginine was achieved by feeding (NH4)2SO4, thus it was 108. 9% higher than that of control.关键词
L-精氨酸/发酵/氮源/重组E.coliKey words
L-arginine/fermentation/nitorgen source/recombinant E. Coli分类
化学化工引用本文复制引用
陈可泉,马江锋,姜岷,韦萍,孙娜亚..氮源对重组大肠杆菌发酵产L-精氨酸的影响[J].生物加工过程,2011,9(6):11-14,4.基金项目
国家重点基础研究发展计划(973计划)资助项目(2011CB707405) （973计划）
国家自然科学基金资助项目(21076105) （21076105）
江苏高校优势学科建设工程资助项目 （）
