生物加工过程2011，Vol.9Issue(6)：35-38,4.DOI:10.3969/j.issn.1672-3678.2011.06.008
十二烷基硫酸钠协同微波-酶法提取布渣叶总黄酮
Extraction of total flavones from Microcos paniculata Linn.by combining sodium dodecyl sulfate with microwave and enzyme
摘要
Abstract
In order to obtain the best method for extraction of total flavones, the uniform design method U12(54 ×3) was used to investigate the effect of microwave extraction time(X1) , sodium dodecyl sulfate concentration(X2), liquid to solid ratio(X3) , enzymatic time(X4) , and water extraction time(.X5) on the yield of total flavones from Microcos paniculata Linn, by combining surfactant with the microwave and the enzyme, a mathematical model was established and the optimal process conditions were obtained with PLS procedure. The optimal extraction parameters of total flavones were as follows: X1 = 1 min, X2 = 0. 16% , X3 =30.75 Ml/g, X4 = 50 min, X5 =30 min. The extraction process had advantages of high-efficiency and safety.关键词
十二烷基硫酸钠(SDS)/酶法/布渣叶/微波/总黄酮Key words
sodium dodecyl sulfate/enzyme/Microcos paniculata Linn/microwave/total flavones分类
医药卫生引用本文复制引用
马婉婉,骆开荣,班颖,刘曦,韩伟..十二烷基硫酸钠协同微波-酶法提取布渣叶总黄酮[J].生物加工过程,2011,9(6):35-38,4.基金项目
国家自然科学基金资助项目(20006003) （20006003）
国家大学生创新计划资助项目(091025114) （091025114）
