生物加工过程2012，Vol.10Issue(1)：1-6,6.DOI:10.3969/j.issn.1672-3678.2012.01.001
响应面优化紫萍黄酮提取工艺及其抗氧化活性
Response surface methodology for optimization of extraction flavonoids from Spirodela polyrrhiza (L.) Schleid and antioxidant evaluation
王桃云 1王金虎 1胡翠英 1顾华杰 1钱玮 1刘成龙1
作者信息
- 1. 苏州科技学院化学与生物工程学院,苏州215009
- 折叠
摘要
Abstract
Response surface methodology was used to optimize microwave-assisted extraction of flavonoids from Spirodelapolyrrhiza (L. ) Schleid. Based on single factor tests, Box-Behnken central composite experiment design was carried out with three correlative factors, I. E. , solid-liquid ratio, ethanol concentration and microwave power. Response surface analysis was used to determine the effect of prime factors on the yield of flavonoids from Spirodela polyrrhiza (L. ) Schleid. Results showed that the yield of flavonoids from Spirodela polyrrhiza (L. ) Schleid was 4. 14%. Under the optimal condition, the solid-liquid ratio was 1:17, the ethanol concentration was 62% and the microwave power was 500 W for 9 min. Antioxi-dant activities were evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical in vitro. The result showed that the DPPH radical scavenging activity of the flavonoids was stronger than that of vitamin C and 2,6-di-tert-butyl-4-methyl phenol on the same concentration.关键词
抗氧化活性/提取/黄酮类/微波/响应面法/紫萍Key words
antioxidant/ extraction/ flavonoids/ microwave/ response surface methodology/ Spirodela polyrrhiza(L. ) Schleid分类
化学化工引用本文复制引用
王桃云,王金虎,胡翠英,顾华杰,钱玮,刘成龙..响应面优化紫萍黄酮提取工艺及其抗氧化活性[J].生物加工过程,2012,10(1):1-6,6.
