生物信息学2011，Vol.9Issue(4)：322-326,330,6.DOI:10.3969/j.issn.1672-5565.2011.04.13
甘蔗几丁质酶基因的电子克隆与生物信息学分析
In silico cloning and characterization of chitinase gene from sugarcane using bioinformatics tools
摘要
Abstract
A novel chitinase gene SCCHI1 from sugarcane (Saccharum officinarum) was cloned in silico based on the corresponding sugarcane EST sequences in NCBI database. Some characters of amino acids encoded by SCCHI1 gene, including the composition of amino acid sequence, physical and chemical properties, transmembrane domain, hydrophobicity/hydrophilicity, subcellular localization, secondary and tertiary structure of protein plus the functional domain, were analyzed by bioinformatics tools. Bioinformatic analysis showed that the full - length SC-CHI1 gene from sugarcane was 1 236 bp and it contained a complete ORF which encoded 329 amino acids. Furthermore, SCCHI1 belonged to glycoside hydrolase family 19 and contained a N -terminal signal peptide, a hinge region and a catalytic domain, which was highly conservative among chitinases from several different plant species. The results above provided some references for experimental cloning of SCCHI1 gene from sugarcane, identification and its application.关键词
甘蔗/几丁质酶基因/电子克隆/生物信息学Key words
Saccharum officinarum/ Chitinase gene/ In silico cloning/ Bioinformatics分类
生物科学引用本文复制引用
苏亚春,李国印,阙友雄,郭晋隆,徐景升,许莉萍..甘蔗几丁质酶基因的电子克隆与生物信息学分析[J].生物信息学,2011,9(4):322-326,330,6.基金项目
现代农业产业技术体系建设专项资金项目(CARS-20) （CARS-20）
国家自然科学基金(30700514和31101196). （30700514和31101196）
