陕西师范大学学报：自然科学版2011，Vol.39Issue(6)：60-63,4.
红色荧光蛋白遗传标记腺病毒衣壳蛋白pⅨ的实验研究
Study on capsid protein p Ⅸ genetically labeled by red fluorescent protein
摘要
Abstract
Adenoviral DNA vector genetically modified with pⅨ-mCherry was successfully constructed by means of homologous recombination in E.coli.Bj5183.The virus lysates were obtained after the transfection of adenovirus plasmid linearized by Pac Ⅰ into HEK293 cells for 7~10 days.Adenoviral lysates were propagated,and then adenoviral particles labeled with pⅨ-mCherry were purified by CsCl density gradient centrifugation.Purified virus particles were examined under fluorescence microscope,and the localization of fluorescently labeled adenovirus was then investigated by applying purified viruses into A549 cells at different time points.The results indicated that adenovirus particles were found to show red fluorescence under fluorescence microscope.With the prolonged incubation time of fluorescently labeled adenovirus with A549 cells,the viral particles were gradually localized around nucleus.关键词
基因治疗/腺病毒/病毒示踪/病毒标记/衣壳蛋白pⅨKey words
gene therapy/adenovirus/virus tracking/labeling of adenovirus/capsid protein pⅨ分类
生物科学引用本文复制引用
赵俊丽,王东阳,南天惠,王丽娴,廖晓玉,夏海滨..红色荧光蛋白遗传标记腺病毒衣壳蛋白pⅨ的实验研究[J].陕西师范大学学报：自然科学版,2011,39(6):60-63,4.基金项目
国家自然科学基金资助项目 （）
陕西师范大学研究生培养创新基金资助项目 （）
陕西师范大学大学生创新基金资助项目 （）
