摘要
Abstract
Objective: The effect of the orchiectomy on the change of ANT] ,GABA expression in hipp-ocamal of pentylenetetrazole induced kindling rats was studyed,in ordered to specul- ate on rats for neutered the influence of epilepsy. Methods: Forty-four healthy male SD rats were randomly divieded into 4 groups: black contrast group(10 rats), saline contrast group (10 rats), epilepsy model group (12 rats), Orchiectomized model group (12rats). The model groups use the sub-eclampsia dosage(35mg/kg)of Pentetra-zole(PTZ) to inject, the change of ANT1 、GABA expression in hippocamal of pentylenete-trazole were observed and the data were statistically analyzed. Results: HE stain :Cells of the hippocampus in the black contrast group and the saline contrast group were normal microscopically. In the epilepsy model group and orchiectomized model group apprared dark and shrunken, with pyknntic nucleus and irregular dispeared chromatin clumps. The ruslts apperad the orchiectomized model group had less acidophilic cells than the epilepsy model group. The observed wim immunohistochemical stain: The nmubers of GABA immunological reacted positive cells were similar in the black contrast group and the saline contrast group, however, it were singnificantly increased in other two groups. The nmubers of GABA immunological reacted positive cells were vere significalltly decreased in epilepsy model groups compared with orchiectomized model groups. 4. The nmubers of ANT1 immunological reacted positive cells had no change in hippocamal of pentylenetetrazole induced kindling rats. Conclusion:The nmubers of ANT1 immunological reacted positive cells had no change in hippocamal of pentylenetetrazole induced kindling rats. 4. Orchiectomized model groups had more espress of GABA in hippocamal, which Consistent with behavior performance.关键词
癫痫/化学诱导/戊四唑/毒性/海马/病理学/γ-基丁酸/代谢/腺嘌呤核苷酸移/位酶/代谢/睾丸切除术/动物,实验/大鼠Key words
Epilepsy/chemically/ Pentylenetetrazole/toxicity/ Hippocampus/pathology/ Gamma-ami-nobutyric acid/metablism/ Adenine nucleotide/ translocase/metablism/ Orchiectomy/ Animals,laboratory/ Rats分类
医药卫生
