陕西医学杂志2012，Vol.41Issue(2)：133-135,195,4.
RvE1对肝缺血再灌注损伤大鼠库普弗细胞中Chemerin表达的影响
Effects of rvel on the expression of chemerin in rats kupffer cells of hepatic ischemia-reperfusion injury
王永光 1李养军 2高小鹏3
作者信息
- 1. 陕西省永寿县人民医院 永寿713400
- 2. 陕西省永寿县人民医院 永寿713400
- 3. 陕西省高陵县人民医院
- 折叠
摘要
Abstract
Objective:To study the expression of chemerin in rats kupffer cells(KCs) of hepatic ischemia-reperfusion injury(IRI) and the effects of resolvin El(RvEi) on it. Methods: seventy-two SD rats divided were randomly into three groups: the sham operation group (control group) , the ischemia-reperfusion group (IRI group) and the RvEi treatment group (RvEi group). A model of partial warm ischemia/reperfusion injury in the rat liver was established. RvEi were given ten minutes before the operation. One hour, six hours, twelve hours and twenty-four hours after the reperfusion, Blood was taken from inferior vena cava to test the hepatic enzyme level, including ALT and AST. Kupffer cells (KCs) were isolated and incubated. Tumor necrosis factor alpha (TNF-a)and interleukin-lbeta (IL-1J3) in the supernatants were measured by enzyme linked immunosorbent assay(ELISA). The expression of chemerin in KCs was detected by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). Results: The ALT and AST level of the RvEi group were significantly lower than those in the ischemia/ reperfusion group(P<0. 01). The mRNA and protein expression of chemerin and the level of TNF-o and IL-l|3in the IRI group significantly increased in the course of the reperfusion and slightly decreased at 24 h, but were still significantly higher than those in the control group (P<0. 05). The expression of these factors were markedly decreased after RvEi treatment compared with the IRI group (P<0. 05). Conclusion: RvEi protect against ischemia/reperfu-sion injury of the rats liver partially by restraining the KCs activation, reducing the excess release of TNF-a and IL-1(3 from KCs and decreasing the high expression of chemerin protein and mRNA.关键词
再灌注损伤/枯否细胞/大鼠/RvE1/ChemerinKey words
Reperfusion injury/Kupffer cells Rats/?Resolvin Ei /?Chemerin分类
医药卫生引用本文复制引用
王永光,李养军,高小鹏..RvE1对肝缺血再灌注损伤大鼠库普弗细胞中Chemerin表达的影响[J].陕西医学杂志,2012,41(2):133-135,195,4.
