中国石油大学学报（自然科学版）2011，Vol.35Issue(6)：159-163,5.DOI:10.3969/j.issn.1673-5005.2011.06.028
响应曲面法优化混合菌KW8-2修复石油污染土壤
Bioremediation condition optimization of crude oil contaminated soil using response surface method with microbial consortium KW8-2
摘要
Abstract
A response surface method was employed to optimize the bioremediation of crude oil contaminated soil by microbial consortium KW8-2. A four-level Box-Behnken factorial design was employed to study the relationship of independent variables and dependent variable by applying initial oil mass fraction, biomass, the ratio of nitrogen and phosphorus, and synthetic surfactant (SDS) as independent variables (factors) and oil removal after60 days remediation as dependent variable (response ). A prediction model of quadramatic polynomial regression equation was obtained. The results show that the crude oil removal of the soil with the oil fraction of 10×l0-3, 30×10-3 and 50×10-3 could reach as high as 67. 12% , 63. 39% and 56. 57% , respectively, compared with 63. 78% , 60. 36% and 55. 44% removal for un-optimized results. The results can provide technical parameters for the crude oil contaminated soil remediation with microbial consortium KW8-2.关键词
环境工程/土壤污染/生物修复/原油/表面活性剂/混合菌Key words
environmental engineering/soil pollution/bioremediation/crude oil/surfactant/microbial consortium分类
资源环境引用本文复制引用
刘春爽,张云波,赵东风,赵朝成,刘其友..响应曲面法优化混合菌KW8-2修复石油污染土壤[J].中国石油大学学报（自然科学版）,2011,35(6):159-163,5.基金项目
中石油科学研究与技术开发项目(2008D-4704-2) （2008D-4704-2）
