实用肝脏病杂志2012，Vol.15Issue(2)：104-107,4.DOI:10.3969/j.issn.1672-5069.2012.02.008
肝复康对肝纤维化大鼠肝组织整合素α5β1/FAK信号通路的调节作用
Effect of Gan-fu-kang compound on hepatic expression of integrinα5β1/FAK signal pathway in carbon tetrachloride-induced liver fibrosis in rats
摘要
Abstract
Objective To study the effect of traditional Chinese medicine Gan-fu-kang (GFK)on hepatic expression of integrinα5β1/ focal adhesion kinase (FAK) in carbon tetrachloride -induced liver fibrosis in rats. Methods SD rats were randomly divided into control, model, low, middle and high dose of GFK treatment groups. Liver fibrosis models of rats were made by subcutaneously injecting with 10%CCl4. GFK was orally given daily with GFK from week 9 to 20. The expression of integrinα5 and integrinβ1 protein and FAK mRNA were detected by immunohistochemical and RT-PCR techniques,respectively. Results The level of integrinα5 expression in model group(0.50±0.01)was obviously increased as compared with that in control group(0.23±0.13,P<0.01 ),and it was obviously downregulated in high,middle and low dose of GFK treatment groups respectively (0.35±0.03,0.33± 0.01,0.38 ±0.02)as compared with model group (P<0.01); Similarly, the level of integrinβ1 expression in model group(0.44±0.02) was obviously increased as compared with that in control group(0.27±0.01 ,P<0.01),and it was decreased in high,middle and low dose of GFK treatment groups respectively (0.37±0.01,0.34±0.01,0.39±0.01) as compared with model group(P<0.01);At the same time,FAK mRNA level in model group(0.73±0.01)was obviously higher than that in control group (0.11±0.02,P<0.01),while it was lower in high,middle and low dose of GFK treatment groups respectively(0.33±0.03,0.28±0.01,0.18±0.01)than that in model group(P<0.01).Conclusion The activated integrinα5β1/FAK signal pathway may play an important role in liver fibrosis,and the therapeutic mechanisms of Gan-fu-kang on hepatic fibrosis may be related to the inhibition of this signal pathway.关键词
肝纤维化/肝复康/整合素α5β1/FAK信号通路/大鼠Key words
Liver fibrosis/Gan-fu-kang/Integrina5β1/FAK signal pathway/Rats引用本文复制引用
张坤,姜妙娜,张彩华,贾玉杰..肝复康对肝纤维化大鼠肝组织整合素α5β1/FAK信号通路的调节作用[J].实用肝脏病杂志,2012,15(2):104-107,4.基金项目
辽宁省自然科学基金资助(编号:201102055) （编号:201102055）
