实用肝脏病杂志2012，Vol.15Issue(2)：126-129,4.DOI:10.3969/j.issn.1672-5069.2012.02.015
肝癌干细胞逃避5-氟尿嘧啶单药杀伤可能是造成化疗失败的重要原因
Liver cancer stem cells escaping from the killing of 5-fluorouracil may lead to chemotherapy failure
摘要
Abstract
Objective To investigate the effect of 5 fluorouracil on the biological characteristics of BEL- 7402 and SMMC-7721 cell line. Methods The proliferation of the two cell lines with 5-FU treatment was detected by cell counting Kit-8; the cell cycle and the ratio of cells with positive expression of tumor stem cell surface markers,EpCAM,ABCG2 and ICAM-1 were detected by flow cytometry. Results The proliferation of the two cell lines at 10μg.ml-1 5-FU treatment was obviously inhibited;the ratio of the rest phase cells(G0/Gl phase) in the experimental group was significantly higher than in the control group (P<0.05). Meanwhile, the proportion of EpCAM,ABCG2 and ICAM-1 positive cells increased as compared to control group(P<0.05). Conclusion Tumor stem cell population in BEL-7402 and SMMC-7721 cell line can escape from the killing effect of 5-FU,which might be a vital important cause of chemotherapy failure in clinic practice.关键词
BEL-7402细胞系/SMMC-7721细胞系/肿瘤干细胞/5-氟尿嘧啶Key words
BEL-7402 cell line/SMMC-7721 cell line/Tumor stem cell/5-fluorouracil引用本文复制引用
李德龙,吕福东,冯骥良,朱雯静,韩旭,杨玥,诸国华,刘宝成,刘菲,康梦田,史亮..肝癌干细胞逃避5-氟尿嘧啶单药杀伤可能是造成化疗失败的重要原因[J].实用肝脏病杂志,2012,15(2):126-129,4.基金项目
北京市教育委员会"人才强教"项目中青年骨干人才培养计划基金(PHR201008387) （PHR201008387）
