实用医学杂志2011，Vol.27Issue(24)：4367-4369,3.DOI:10.3969/j.issn.1006-5725.2011.24.007
人增生性瘢痕成纤维细胞的改良培养和鉴定
Refined culture and identification of human hypertrophic scar fibroblasts
摘要
Abstract
Objective To investigate an easy and short cycle method of culturing hypertrophic scar fibroblasts in vitro. Methods Isolation and culture of hypertrophic scar fibroblasts were administered by the method which combined tissue explants adherent and enzyme digestion. The morphological changes of fibroblasts were observed under inverted microscope, then the fibroblasts were identified with immunocytochemistry, and the proliferation assay was performed with growth curve. Results The culture cycle of the method which combined tissue explants adherent and enzyme digestion was short. The expression of vimentin protein was positive in fibroblasts cultured. The subcultured fibroblasts possessed a strong reproductive ability. Conclusion Stable fibroblasts were cultured more easily and quickly with the method combining the tissue explants adherent and trypsin digestion.关键词
成纤维细胞/增生性瘢痕/体外培养Key words
Fibroblast/Hypertrophic scar/Culture in vitro引用本文复制引用
林尊文,徐少宏,游敏,刘德伍,钟世镇..人增生性瘢痕成纤维细胞的改良培养和鉴定[J].实用医学杂志,2011,27(24):4367-4369,3.基金项目
国家自然基金资助项目(编号:81060323) （编号:81060323）
