实用医学杂志2012，Vol.28Issue(3)：367-369,3.DOI:10.3969/j.issn.1006-5725.2012.03.011
辛伐他汀对大鼠血管平滑肌细胞增殖及FGFR1与p21ras表达的干预作用
Effect of simvastatin on the proliferation and the expression of FGFR1 and p21ras in rats vascular smooth muscle cells
摘要
Abstract
Objective To investigate the effect of simvastatin on the proliferations and the expressions of FGFR1 and p21ras in rats vascular smooth muscle cells (VSMCs). Methods VSMCs were cultivated in vitro and VSMC models of proliferation were established in the study. The alive cells counting by tetrazolium dye-reduction assay (MTT) were use to determine the effect of simvastatin on VSMCs proliferation at different concentrations (0, 1, 5, 10 μmol/L), the expressions of FGFR1 and p21ras were detected by Western blot methods. Results Compared with the control group, other simvastatin concentration groups were appeared different levels of growth inhibition (P < 0.05); Compared with the control group, the expressions of FGFR1, p21ras was unconspicuously changed in low dose group while the expressions were more significantly reduced in middle dose and high dose groups (P < 0.05); Compared with middle dose group, the expression was clearly reduced in high dose group group (P < 0.05), and there was a obvious dose dependency. Conclusions Simvastatin might play a role of inhibiting VSMCs proliferation by reducing the expression of FGFR1 and p21ras, it may be one of the important mechanisms that simvastatin treated cardiovascular disease such as atherosclerosis, restenosis and hypertension.关键词
辛伐他汀/VSMC/FGFR1/p21rasKey words
Simvastatin/ Vascular smooth muscle cell/ FGFR1/ p21ras引用本文复制引用
侯慧芳,刘国庆,王永玲,孙银平,郭勇..辛伐他汀对大鼠血管平滑肌细胞增殖及FGFR1与p21ras表达的干预作用[J].实用医学杂志,2012,28(3):367-369,3.基金项目
河南省教育厅自然科学基金资助项目(编号:2008A310012) （编号:2008A310012）
河南省科技厅科技攻关课题基金资助项目(编号:0424410129) （编号:0424410129）
