实用医学杂志2012，Vol.28Issue(4)：537-539,3.DOI:10.3969/j.issn.1006-5725.2012.04.006
血卟啉单甲醚对人宫颈癌Hela细胞体外影响的研究
Effects of hematoporphyrin monomethyl ether on human cervical carcinoma Hela cell lines
摘要
Abstract
Objective To investigate the lethal effects of hematoporphyrin monomethyl ether (HMME) mediated photodynamic therapy (PDT) on human cervical carcinoma Hela cell lines and the factors affecting these effects. Methods Hela cells were treated with different concentrations of HMME, different energy density of laser, and different incubation time, then the survival rate of Hela cells was measured by MTT assay 24 h later. Results There were significant differences of cell growth inhibition among the groups with concentrations of HMME less than 2.5 μg/ mL, but there were no significant differences among the groups with laser energy density more than 1.5 J/cm2 and incubation time more than 4 h. The parameters of 2.5 μg/mL of HMME, 1.5 J/cm2 of laser energy density, and more than 4 h of incubation time were the best for the lethal effects of HMME-PDT on Hela cells. Conclusions The main impact factors of HMME-PDT include concentrations of HMME , laser energy density, and incubation time.关键词
宫颈肿瘤/血卟啉单甲醚/光动力疗法Key words
Uterine cervical neoplasms/Hematoporphyrin monomethyl ether/Photodynamic therapy引用本文复制引用
蔡思娜,罗荣城,梁卫江,李黎波,张兰英,陈晓华,张志锋..血卟啉单甲醚对人宫颈癌Hela细胞体外影响的研究[J].实用医学杂志,2012,28(4):537-539,3.基金项目
广东省自然科学博士启动基金(编号:S2011040000786) （编号:S2011040000786）
