实用医学杂志2012，Vol.28Issue(4)：564-567,4.DOI:10.3969/j.issn.1006-5725.2012.04.016
FMR1基因敲除对雄性小鼠生殖功能的影响
The influence of FMR1 gene knockout on the reproduction of male mice
祝亚桥 1周兴 1陈盛强2
作者信息
- 1. 510260,广州医学院第二附属医院泌尿外科
- 2. 510260,广州医学院第二附属医院神经科学研究所
- 折叠
摘要
Abstract
Objective To investigate the influence of fragile x mental retardation-1 (FMR1) gene on the spermatogenesis and reproduction of male mice. Methods FMR1 knockout (KO) male mice and wild type (WT) male mice were mated with wt female mice. The number of litters, pregnancy rate and male mice having offsprings were counted. Serum T, FSH and LH concentrations were also measured. The density, mortality and morphology of the left cauda epididymis sperms were analyzed, HE staining was performed on the right side. Results The pregnance rate of wt female mice mated with FMR1K0 males was significantly lower than the control group (41.7% vs. 87. 5% , P < 0.05) ,the average litter size was 6.50 ± 2.27 and 8.22 ± 3.03 (P > 0.05). Male fertility showed that 41.7% of KO mice had pups, whereas 91.7% of the mice had pups in the control group (P < 0.05). No obvious pathological difference in testes and epididymides was found between the two groups. There was no significant difference in the sperm count, motility and abnormal sperm ratio in two groups. Conclusions FMR1 gene may play an important role in the development of male genital system and the absence of FMR1 reduce the reproduction of male mice. The mechanisms of FMR1 gene influencing the male genital system are needed to further study.关键词
脆性X智力低下蛋白质/基因敲除/生殖/精子发生Key words
Fragile X mental retardation protein/Knockout/Reproduction/Spermatogenesis引用本文复制引用
祝亚桥,周兴,陈盛强..FMR1基因敲除对雄性小鼠生殖功能的影响[J].实用医学杂志,2012,28(4):564-567,4.
