实用医学杂志2012，Vol.28Issue(4)：576-578,3.DOI:10.3969/j.issn.1006-5725.2012.04.021
皮肤病理性瘢痕和瘢痕癌中Ras基因家族12/13位密码子突变的检测
Mutation of ras gene family codons 12 and 13 in skin pathological scar and scar carcinoma tissues
摘要
Abstract
Objective To explore the mutation of Ras gene family (H-ras/N-ras/K-ras) in skin pathological scar and scar carcinoma tissues. Methods The DNA was extracted from the choosen samples fixed in 10% formalin and Paraffi embedded (including 14 cases skin pathological scar tissues and 14 cases skin scar carcinoma tissues) ,H-ras,N-ras and K-ras mutations in codons 12 and 13 were analyzed by PCR and sequencing. Results DNA was extracted from the choosen 28 samples. By PCR, the 28 samples were succeed to enlarge the gene piece.Without gene mutation in codons 12 and 13 of H-ras,N-ras and K-ras genes were examined by sequencing. Conclusions The mutation situs of Ras family is to be further explored in skin scar and scar carcinoma.关键词
突变/Ras基因/皮肤病理性瘢痕/瘢痕癌Key words
Mutation/Ras gene/Skin pathological scar/Scar carcinoma引用本文复制引用
胡成久,郭瑞珍,王燕..皮肤病理性瘢痕和瘢痕癌中Ras基因家族12/13位密码子突变的检测[J].实用医学杂志,2012,28(4):576-578,3.基金项目
贵州省攻关项目(编号:2010-3080) （编号:2010-3080）
