实用肿瘤杂志2012，Vol.27Issue(1)：13-17,5.
NF-κB介导LMP1在鼻咽癌干细胞SP18增殖中的作用
Effects of latent membrane protein 1 mediated by nuclear factor-κB on proliferation of nasopharyngeal cancer stem cell SP18
摘要
Abstract
Objective To investigate the proliferation mechanism of nasopharyngeal cancer stem cell SP18 affected by carboxy terminal activating region-2 of latent membrane protein 1. Methods The method of retroviruses infection was adopted and the nasopharyngeal cancer stem cell SP18 of stable expression LMP1 and LMP1TRADD (pLNSX vector was negative control) was established. Then,the growth of nasopharyngeal cancer stem cell SP18 affected by LMP1TRADD was observed by using cell growth curve,plate clone formation, soft agar colony and flow cytometry. Meanwhile, the expression of NF-kB protein was detected by Western-blot in SP18-LMP1TRADD cell and SP18-LMP1 cell. Results (1) Compared with SP-pLNSX cell,the growth velocity and proliferation index of SP18-LMPlcell obviously increased (n=3,P<0.01). (2) Compared with SP18-LMP1 cell, the growth velocity and proliferation index of SP18-LMP1TRADD cell obviously decreased ( n = 3 ,P < 0. 01). ( 3 ) The expression of NF-kB protein in SP18-LMP1TRADD cell was lower than in SP18-LMP1 cell,similar with SP-pLNSX cell. Conclusion Carboxy terminal activating region-2 of LMP1 probably regulats the expression of NF-kB protein in SP18 to decrease cellular proliferation index and affect the cellular growth and proliferation.关键词
鼻咽肿瘤/LMP1/干细胞/肿瘤细胞,培养的/细胞增殖/NF-κB蛋白/流式细胞术Key words
nasopharyngeal neoplasms/ latent membrane protein 1/ stem cells/ tumor cells, cultured/ cellular proliferation/ NF-kB protein/flow cytometry分类
医药卫生引用本文复制引用
沈华,张志伟,刘重元,赵强,朱建思,董琳,罗招阳..NF-κB介导LMP1在鼻咽癌干细胞SP18增殖中的作用[J].实用肿瘤杂志,2012,27(1):13-17,5.基金项目
湖南省高校创新平台开放基金(10K052) （10K052）
湖南省重点学科建设基金(2006-180) （2006-180）
湖南省研究生科研创新项目(CX2010B380) （CX2010B380）
湖南省大学生研究性学习和创新性实验计划项目(CXSY-SJ-09007) （CXSY-SJ-09007）
