天津中医药2011，Vol.28Issue(6)：486-488,3.
小半夏加茯苓汤含药血清对小鼠胚胎干细胞体外增殖及分化的影响
Effect of Xiaobanxia added Fuling decoction-containing serum on the proliferation and differentiation of mouse embryonic stem cells in vitro
摘要
Abstract
[Objective] To investigate the effect of Xiaobanxia added Filling decoction-containing serum on the proliferation and differentiation of mouse embryonic stem cells (ESCs) in vitro. [Methods] Isolated ESCs were cultured in vitro. The cells were treated with drug-containing serum and Qingbanxia respectively, the blank serum and fetal bovine serum were used as the control. The proliferation of cells was detected by MTT assay at 24, 48, 72 and 96 h. The differentiation of cells to cardiac myoblasts specific genes (β-MHC) was detected by RT-PCR. [Results] Mouse ESCs were proliferated after treating with drug-containing serum at 24, 48, 72 and 96 h. Differentiation of mouse ESCs were good when treated with this serum. Compared with control group there were no significant differences. (ft>0.05). [Conclusion] Xiaobanxia added Fuling decoction-containing serum tan promote ESCs proliferation, and effectively stimulate ESCs differentiation into cardiac myoblasts. It is possible that Xiaobanxia added Fuling decoction may not have any embryotoxicity.关键词
小半夏加茯苓汤/胚胎干细胞/增殖/分化/胚胎毒性Key words
Xiaobanxia added Fuling decoction/embryonic stem cell/proliferation/differentiation/embryotoxicity分类
医药卫生引用本文复制引用
宋殿荣,王雅楠,张崴,朱泽,鄣洁..小半夏加茯苓汤含药血清对小鼠胚胎干细胞体外增殖及分化的影响[J].天津中医药,2011,28(6):486-488,3.基金项目
中国博士后科学基金资助项目(20080430739). （20080430739）
