天津中医药2011，Vol.28Issue(6)：489-492,4.
肝气郁结证动物模型的建立及HPA轴相关激素的初步研究
Establishment of syndrome of stagnation of liver Qi model in rats and primary study on related hormones of hypothalamus-pituitary-adrenal axis
摘要
Abstract
[Objective] To establish and validate the syndrome of stagnation of liver Qi model in rats. The change of hormones related to hypothalamus-pituitary-adrenal (HPA) axis was investigated. [Methods] Twenty rats were randomly divided into two groups: control group and model group. We adopted the method of chronic stress combining with isolated breeding to prepare the model in rats with the stagnation of liver-Qi syndrome. Open-Field test and partial addicted to sugar water test were carried out one the day before experiments and after experiments. The rat's body weight and food intake in 24 h were recorded on the day before experiments, and on the 4th , 8th, 12th, 16th, 20th, 24th and 28th day during experiments respectively. The content of adrenocorticotropic hormone-releasing hormone (CRH) and serum corticosterone (CORT) were measured by ELISA method. [Results] There were some appearance of abnormal behaviors, such as decrease of elusion, slackness, looser stools, and matte fur among model rats. After two weeks the body weight of model group was significantly lower than that of control group (P<0.05). After chronic stress procedure for 4 weeks, both horizontal movement scores and vertical movement scores of rats in model group were significantly reduced compared with the normal group (P<0.01). Sugar consumption, the degree of partial addicted to sugar water and food intake of model group rats decreased significantly (P<0.01). The serum contents of CRH and CORT in model group rats were significantly higher compared with the control group (P<0.01). [Conclusion] Based on the etiological, behavioral and pathological observation, it can be concluded that the rat model of syndrome of stagnation of liver Qi was successfully established by the method of chronic stress combining with isolated feeding. The rats with stagnation of liver-Qi syndrome shows a state of hyperactivity of HPA axis, which can help to explain the essence of its pathologic changes.关键词
肝气郁结证/大鼠/HPA轴Key words
stagnation of liver-Qi syndrome/rat/hypothalamus-pituitary-adrenal axis分类
医药卫生引用本文复制引用
赵海滨,刘子旺,张秀静..肝气郁结证动物模型的建立及HPA轴相关激素的初步研究[J].天津中医药,2011,28(6):489-492,4.基金项目
国家自然科学基金项目(81072772) （81072772）
首都医学发展科研基金项目(SF-2007-Ⅲ-06). （SF-2007-Ⅲ-06）
