天津中医药2011，Vol.28Issue(6)：493-496,4.
滋肾调冲法对大鼠外周血内皮祖细胞影响的实验研究
Effect of Zishentiaochong therapy on peripheral blood endothelial progenitor cells in rats
摘要
Abstract
[Objective] To study the effect of Gongxuening D prescription, a representative of Zishentiaochong therapy on the number of peripheral blood endothelial progenitor cells (EPCs) in rats transplanted with single marrow nuclear cells and explore the mobilization effect on EPCs. [Methods] Female Wistar rats with bone marrow cells transplantation were taken as experimental animals. Each of them was prepared by injecting bone marrow mononuclear cells suspension into the tail veins within 6 h after a general irradiation by 137Cs. The bone marrow mononuclear cells were conventionally extracted and separated from the bilateral femoral marrows of adult male Wistar rats under aseptic conditions and fluorescence labeled by 4, 6- two acetyl-2-phenyl indole (DAPI ). The experimental female rats with the transplantation were randomly divided into three groups and some normal female Wistar rats with a infusion of equivalent blank medium via tail vein were taken as the fourth group. After 24 h of the bone marrow transplantation, the first group rats with the transplantation was intragastrically given physic liquor of Gongxuening Ⅱ as Gongxuening II group, the second group rats was given isometric physic liquor of WujiBaifeng pills as WujiBaifeng Wan group, both the third and the fourth groups were given isometric physiological saline as transplantation control group and pseudopregnancy control group. After hematopoietic reconstitution in the rats with the transplantation, all the rats of the three groups and the fourth normal control group were prepared the pseudopregnancy model by using PMCG + HCG. At the early, medium and advanced luteal stages 1/3 of each group were killed for getting intraocular canthal blood, making blood smears , observing the distribution of DAPI staining cells in the blood by fluorescence microscopy and detecting peripheral blood EPCs number by flow cytometry. [Results] It was revealed by fluorescence microscopy that in the early stage a larger number of DAPI staining cells can be found in blood smears made from transplanted rats, especially from the rats of Gongxuening II group. In all groups, the number of peripheral blood EPCs was maximum in early luteal stage and slightly reduced in medium luteal stage. The number of peripheral blood EPCs in advanced luteal stage was significantly reduced compared with the results in early or medium luteal stage (P<0.05 orP<0.01). In every stage the numbers of peripheral blood EPCs from the both Gongxuening II group and WujiBaifeng Wan group were obviously higher than those from pseudopregnancy control group (P<0.05 or P<0.01). In the early stage the number of EPCs from Gongxuening II group was significantly increased compared with the numbers of EPCs from WujiBaifeng Wan group and transplantation control group (P<0.05). [Conclusion] Zishentiaochong therapy can increase the number of circulating blood EPCs through mobilizing bone marrow EPCs into peripheral blood. It is important to home EPCs to the corpus luteum and promote luteal angiogenesis.关键词
滋肾调冲法/功血宁Ⅱ号方/动员/内皮祖细胞/骨髓单个核细胞Key words
Zishentiaochong therapy/Gongxuening II/ mobilize/ endothelial progenitor cells/bone marrow mononuclear cells分类
医药卫生引用本文复制引用
宋春敬,刘晓帆,孙艳明,李广斌,陈静,李海霞,李瑞环,王玲..滋肾调冲法对大鼠外周血内皮祖细胞影响的实验研究[J].天津中医药,2011,28(6):493-496,4.基金项目
国家自然科学基金资助项目(30873281). （30873281）
