天然产物研究与开发2012，Vol.24Issue(1)：79-83,5.
多糖的甲基化方法及图谱解析
Method and Analysis of Methylation of Polysaccharides
摘要
Abstract
Methylation of trace polysaccharide was studied. .Polysaccharide sample (0. 1 mg) was dissolved in 0. 3 mL dimethyl sulfoxide,followed by adding 10 mg powdered NaOH and sonicating for 20 min. After cooling on ice,0. 1 mL CH3I was added and sonicated for 20 min at 18-20 ℃. After cooling,CH31 was added again and sonicated for 20 min. Water (1 mL) containing 4 mmol/L Na2S2O3 was added to stop the methylation reaction. The. Methylated solution was extracted four times with 0.5 mL chloroform,followed by extracted five times with 0.5 mL water. The chloroform fraction was mixed with anhydrous sodium sulfate to remove the residue water and dried with N2. The method was simple and easy. The methylation degree of the product was high for polysaccharides soluble or insoluble in water and DMSO. In addition, the resolution of partially methylated alditol acetate was explained and the methylation methods for special carbo-hydrates were also illuminated.关键词
多糖/甲基化/方法/图谱解析/变形链球菌/鸡腿菇Key words
polysaccharide/ methylation/ method/ spectrum elucidation/ Streptococcus mutans/ Coprinus comatus分类
生物科学引用本文复制引用
李波,芦菲..多糖的甲基化方法及图谱解析[J].天然产物研究与开发,2012,24(1):79-83,5.
