基质金属蛋白酶-1shRNA真核表达载体的构建OACSTPCD
Construction of eukaryotic expression vectors of short hairpin RNA for MMP1
目的 构建基质金属蛋白酶-1(MMP-1)特异的RNA干扰质粒载体.方法 根据GenBank 数据库提供的MMP-1基因核苷酸序列,选择设计能转录短发夹状RNA (Short hairpin RNAs,shRNA) 的DNA 序列,并与pWH1质粒载体连接,用酶切的方法 进行鉴定;将构建成功的特异性表达载体(pWH1-MMP1)稳定转染至人腺样囊性癌ACC-M细胞系后,应用RT-PCR、免疫组织化学及Western Blot对细胞MMP1的表达进…查看全部>>
Objective To construct eukaryotic expression vectors of RNA interference specific to matrix metalloproteinase 1 ( MMP1) . Methods Genome sequences of MMP1 gene were retrieved from Genbank and cDNA that was capable of coding expression of shRNA (small hairpin RNAs) was designed for MMP1 gene. The cDNA was synthesized and inserted into plasmid pWHl, and the recombinant pWHl - MMP1 expression vector was identified using enzyme cutting. Then, pWHl - MMP1 express…查看全部>>
朱秀丽;朱晓英;郭青玉;王静;温德升;吴军正
100039,北京,武警总医院口腔科100039,北京,武警总医院口腔科710032,西安,第四军医大学口腔医院口腔生物教研室710032,西安,第四军医大学口腔医院口腔生物教研室710032,西安,第四军医大学口腔医院口腔生物教研室710032,西安,第四军医大学口腔医院口腔生物教研室
基础医学
涎腺腺样囊性癌短发夹状RNA真核表达载体基质金属蛋白酶1
salivary gland adenoid cystic carcinoma short hairpin RNA eukaryotic expression vector matrix met all op rot ei-nase 1
《武警医学》 2012 (2)
用RNA干扰技术抑制人涎腺癌细胞增殖和转移
111-114,4
国家自然科学基金资助项目(30371551)
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