武警医学2012，Vol.23Issue(2)：150-152,3.
脂血和溶血对HBV DNA实时荧光定量PCR检测的影响
Influence of hemolysis and lipoidemia on HBV DNA real-time PCR
张翠莉 1刘萍 2魏文波1
作者信息
- 1. 614000,乐山,武警四川总队医院检验科
- 2. 610041,武警四川总队成都分院检验科
- 折叠
摘要
Abstract
Objective To study the influence of hemolysis and lipoidemia on real -time fluorescence quantitative polymerase chain reaction ( RT - PCR) of HBV DNA. Methods (1) Clinical HBV DNA specimens were collected, and were divided into level I and level II according to HBV DNA concentrations. HBV DNA negative but severe blood lipid samples and normal blood samples were collected. These specimens were mixed in different proportions and divided into group A, B, C by different TG concentrations. (2) Clinical HBV DNA specimens were collected, three tubes of blood were obtained from each person, and two of them were frozen in a -20 °C refrigerator for different lengths of time to make hemolytic samples of different degrees. Then, they were divided into group D, E, F according to the degree of hemolysis. Results There is no statistically significant difference between the lipoidemia and hemolysis groups in the measurement of HBV DNA RT-PCR (P>0.05). Conclusions Lipoidemia and hemolysis do not affect the results of HBV DNA RT-PCR.关键词
脂血/溶血/实时荧光定量PCR/HBV DNAKey words
lipoidemia/ hemolysis/ RT PCR/ HBV DNA分类
医药卫生引用本文复制引用
张翠莉,刘萍,魏文波..脂血和溶血对HBV DNA实时荧光定量PCR检测的影响[J].武警医学,2012,23(2):150-152,3.
