外科理论与实践2011，Vol.16Issue(6)：562-567,6.
下调MicroRNA-21对胃癌细胞生物学行为及PTEN表达的影响
Biological behaviour and PTEN expression change after MicroRNA-21 down-regulation in gastric cancer cells
张保贵 1刘炳亚 2燕敏 3陈雪华 2俞焙秦 3朱正纲1
作者信息
- 1. 上海交通大学医学院附属瑞金医院 外科
- 2. 上海交通大学医学院附属瑞金医院上海消化外科研究所
- 3. 上海交通大学医学院附属瑞金医院 上海市胃肿瘤重点实验室,上海200025
- 折叠
摘要
Abstract
Objective To investigate the effective relationship between the MicroRNA-21 (miR-21) down-regulation and the biological behaviour and FTEN expression change in gastric cancer, and the possible mechanisms of miR-21 in gastric cancer initiation and development. Methods Transfect the gastric cancer cell lines that miR-21 high expression validated previously by the research team with miR-21 inhibitor; the influence on the proliferation, apoptosis, cell cycle and migration in gastric cancer cell lines were evaluated by CCK-8 kit and Annexin-V+PI and Transwell test; and to evaluate the change of PTEN expression after miR-21 depression in gastric cancer cell lines with Western blotting and luciferase reporter assay. Results The status of cell proliferation in vitro showed that the down-regulation of miR-21 produced an obvious inhibitive effect on the gastric cancer cell proliferation (P<0.05); in vitro experiments also showed that miR-21 depression promoted apoptosis of gastric cancer cell lines of miR-21 high expression (P<0.05), mainly G1/S in the cell cycle (P<0.05); Transwell test showed that cell migration were notablely inhibited (P<0.05); the Western blotting and luciferase reporter assay also showed that PTEN expression increased remarkably after miR-21 inhibition (P< 0.05). Conclusions Down-regulation of miR-21 has a strong inhibitory effect on proliferation in the gastric cancer cell lines of miR-21 high expression, and promotes apoptosis of these cells; meanwhile, depresses migration in vitro; and miR-21 down-regulation up-regulates the PTEN expression level; so PTEN may be a target gene in gastric cancer initiation and development.关键词
miR-21/胃癌/生物学行为/PTENKey words
miR-21/Gastriccancer,Biologicalbehaviour,PTEN分类
医药卫生引用本文复制引用
张保贵,刘炳亚,燕敏,陈雪华,俞焙秦,朱正纲..下调MicroRNA-21对胃癌细胞生物学行为及PTEN表达的影响[J].外科理论与实践,2011,16(6):562-567,6.
