外科理论与实践2011，Vol.16Issue(6)：576-580,5.
散发性乳腺癌中BRCA1基因甲基化状态与其mRNA表达水平的相关研究
Study in BRCA1 gene methylation status and mRNA expression level in sporadic breast cancer
摘要
Abstract
Objective To investigate the relationship between the abnormal methylation condition of BRCA1 gene in sporadic breast cancer and the changes in the expression level of its Mrna in the region of Shandong peninsula; study in the abnormal epigenetical role of BRCA 1 gene in sporadic breast cancer. Methods To research the methylation status of BRCA1 gene promoter and the changes in the expression level of its Mrna in breast cancer tissue, corresponding adjacent normal tissue and benign breast tumor tissue with the methylation-specific PCR and RT-PCR methods. Results In 30 cases with sporadic breast cancer, the methylation rate of BRCA 1 gene was 43%, and the decrease in expression rate was 33%; the expression rate dropped to 35% in 13 cases with cancerous tissue samples with BRCA1 methylation, while the rate of unmethylated cases (17 cases) was 18%. In relation to the classification of the tumor, the BRCA 1 methylation rate was 74% in the tumor tissue of cases belonging to grade T3, which was higher than the total rate of 26% in grades of T1 and T2, while the Mrna expression rate of decline (54%) was also higher than the total rate of 30% in grades T1 and T2 patients. The BRCA 1 methylation rate (61%) and the declination rate of Mrna expression in patients with lymph node metastasis (46%) were higher than both the corresponding rates (17% and 16%) in patients without lymph node metastasis. Conclusions The declination or inactivation of BRCA1 Mrna expression may be due to the epigenetic change in the BRCA 1 gene, which is closely related to the grade of malignancy in sporadic breast cancer and the associated lymph node metastasis.关键词
BRCA1基因/DNA甲基化/逆转录-聚合酶链反应/甲基化特异性/散发性乳腺癌Key words
BRCA1gene/DNAmethylation/RT-PCR/Methylation-specificpolymerasechainreaction/Sporadicbreastcancer分类
医药卫生引用本文复制引用
吴琍,曹伟红,陈庆峰,郑玲莉,吴艳艳..散发性乳腺癌中BRCA1基因甲基化状态与其mRNA表达水平的相关研究[J].外科理论与实践,2011,16(6):576-580,5.基金项目
山东省自然科学基金(009ZRB 14999) （009ZRB 14999）
