西安交通大学学报（医学版）2012，Vol.33Issue(1)：5-10,6.
PPAR-γ激活对血管平滑肌细胞外基质释放及结缔组织生长因子表达的影响
Effects of PPAR-γ activation on angiotensin Ⅱ-induced extra cellular matrix production and CTGF expression in VSMCs
摘要
Abstract
Objective To observe the effects of PPAR-y activation on angiotensin (Ang) U -induced extra cellular matrix (ECM) production and connective tissue growth factor (CTGF) expression in vitro in rat vascular smooth muscle cells (VSMCs). Methods Cultured rat VSMCs were pretreated with PPAR-y activator rosiglitazone or/and PPAR-y antagonist GW9662 or BADGE before treated with Ang II for 24 h. The content of hydroxyproline in the cell culture medium was measured by colorimetric assay. Real-time RT-PCR was used to detect collagen H (Col III ), fibronectin (FN) and CTGF expression. Protein expressions of CTGF and PPAR-y were measured by Western blotting. ELISA-based DNA-binding was used to detect PPAR-y activation. Results Ang [I treatment up-regulated PPAR-y expression but significantly decreased its activity when compared with control group. Pretreatment of cells with rosiglitazone significantly increased PPAR-y expression and activation in Ang H -stimulated VSMCs. Rosiglitazone decreased hydroxyproline level in cell culture medium, down-regulated Col IE , FN and CTGF expressions in VSMCs. Other PPAR-y activators had the same effect as rosiglitazone, but PPAR-y antagonist partly reduced this effect. Conclusion PPAR-y activation may inhibit CTGF expression and ECM production in VSMCs. PPAR-y might act as a therapeutic target for vascular fibrosis.关键词
血管紧张素/细胞外基质/结缔组织生长因子(CTGF)/PPAR-γ/大鼠/血管平滑肌(VSMCs)/血管纤维化Key words
angiotensin/ extra cellular matrix/ connective tissue growth factor (CTGF)/ PPAR-y/ rat/ vascular smooth muscle cells (VSMCs)/ vascular fibrosis分类
医药卫生引用本文复制引用
高登峰,牛小麟,郝广华,魏瑾,孟哲..PPAR-γ激活对血管平滑肌细胞外基质释放及结缔组织生长因子表达的影响[J].西安交通大学学报（医学版）,2012,33(1):5-10,6.基金项目
国家自然科学基金资助项目(No.30900617,No.81070219),教育部博士点基金新教师基金资助项目(No.2008.6981036),陕西省科技攻关项目(No.2007K13-03(13)),国家重点研究发展基础计划(973)(No.2011CB503904) （No.30900617,No.81070219）
