西北林学院学报2012，Vol.27Issue(1)：81-84,4.DOI:10.3969/j.issn.1001-7461.2012.01.16
枣未成熟胚高效再生体系研究
An Efficient System for Plant Regeneration from Immature Embryo of Chinese Jujube
摘要
Abstract
An efficient system for plant regeneration from immature embryo of Chinese jujube was established by u-sing two callivars of male sterile No. 3CJMS3) and 'Jinmangguozao'. The results showed that the plant regeneration could be achieved by two types of procedures: direct embryo germination and generation of callus of cotyledon. Cold treatment could efficiently accelerate the direct plant regeneration from immature embryo. The plant regeneration rate of immature embryos, which were kept at 0~4℃ for 60 days and then inoculated in medium MS + sugar 30 g ? L-1 + agar 3. 3 g ? L-1, was as high as 93. 3%. Callus could be induced from 98. 9% of cotyledons damaged during smashing stones in medium MS +maltose 20 g ? L-1 +agar 3. 3 g ? L-1 +TDZ 1. 0 mg ? L-1 +NAA 0. 2 mg ? L-1 within 40 days. Adventitious buds occurred in 74.1% of the callus cultured in medium MS + maltose 20 g ? L-1 +agar 3. 3 g ? L-1 +TDZ 1. 0 mg ? L-1 for 40 days with cluster buds rate of 70. 4%. 1/2MS + sugar 20 g ?. L-1 + agar 3. 3 g ? L-1 + IBA 1. 0 mg ? L-1 + IAA 0. 01 mg ? L-1 was proper rooting medium and the rooting rate reached 80. 8% in 40 days.关键词
枣/未成熟胚/子叶/植株再生Key words
Chinese\I'/\ibe(Ziziphus jujuba)/ immature embryo/ cotyledon/ plant regeneration分类
农业科技引用本文复制引用
崔秀梅,王玖瑞,刘孟军,代丽,郭敬丽..枣未成熟胚高效再生体系研究[J].西北林学院学报,2012,27(1):81-84,4.基金项目
国家自然科学基金资助项目(30471194) （30471194）
河北农业大学博士科研补助资金项目. （）
