西北师范大学学报（自然科学版）2011，Vol.47Issue(6)：69-74,6.
热休克诱导虹鳟三倍体的最佳条件
The best condition to induce triploid rainbow trout(Oncorhynchus mykiss) by heat shock
摘要
Abstract
Heat shock treatment is utilized to deal with eggs of rainbow trout forming triploid, which by the retention of the second polar body of fertilized eggs. Based on eyed rate and triploidy rate for the analysis of variance comparison, three factors (treatment temperature, treatment processing time, treatment processing duration) are selected to investigate. The result shows that using heat shock treatment induced triploid rainbow trout and three temperatures are all successfully induced the triploid rainbow trout. As the shock temperature, the dead rate of fertilized eggs has increased within 2 h. In the 30 °C and treatment time lasts longer than l0min conditions rainbow trout eggs all died or stop the growth. So 30 °C is very close to the lethal temperature rainbow trout eggs. In other conditions, as the extension of treatment duration, the death rate of fertilized eggs has increased within 2 h. An effective procedure for inducing triploid is putting the fertilized egg into the 26. 5 °C after 15min insemination to heat shock 10 min. Under that situation, about 83. 18% triploidy embryos is obtained. It leads to conclusions that the most important factor for triploidy induction is duration time, the second is temperature and the third is initiative time of shock.关键词
虹鳟/三倍体/热休克/倍性检测Key words
Oncorhynchus mykiss/ triploid/ heat shock/ ploidy detection分类
生物科学引用本文复制引用
张艳萍,王太,俞小牧,娄忠玉,焦文龙..热休克诱导虹鳟三倍体的最佳条件[J].西北师范大学学报（自然科学版）,2011,47(6):69-74,6.基金项目
甘肃省科技支撑计划项目(097JKCA080) （097JKCA080）
甘肃省重点实验室建设计划(1009RTSA006) （1009RTSA006）
