现代妇产科进展2011，Vol.20Issue(11)：877-880,885,5.
VEGF-C对宫颈癌细胞增殖和凋亡信号传导通路的影响
Study on the cell signal transductions of cells proliferation and apoptosis induced by VEGF-C in cervical carcinoma
杜雪 1糜若然1
作者信息
- 1. 天津医科大学总医院妇产科,天津300052
- 折叠
摘要
Abstract
Objective:To investigate cancer cells proliferation and apoptosis regulated by VEGF-C on HeLa cell line. To confirm whether KI)R,a receptor of VEGF-C, and cell trans-duction pathways PI3K or MAPK were involved in the proliferation and apoptosis induced by VEGF-C in vivo. Methods; Stimulated by recombinant VEGF-C in vivo, HeLa cells were used to analyze the index of cell proliferation by MTT assay. Early apoptosis and cell cycle were analyzed by FCM and proteins expression of Bcl-2 and Cyclin Dl by Western Blotting. HeLa cells were treated by VEGF-C,KDR antibody,MAPK specific inhibitor PD98059,and PI3K specific inhibitor LY294002,and the effects were the investigated through the above-mentioned applications. Results:Treatment of VEGF-C(50ng/(xl) was found to increase cells proliferation (2. 13 vs 1.00),cells number in S phase(64. 26±0. 20 vs 30.91±0.09) ,and apoptosis index significantly reduced after the stimulation of VEGF-C(5. 5±0. 25 vs 12. 4±0. 30). The protein expression level of Bcl-2 and Cyclin Dl in HeLa were increased after stimulated by VEGF-C. The effects induced by VEGF-C including cells proliferation,apoptosis inhibition,the Bcl-2 and Cy- clin D1 expression were blocked in certain degree by Pre-simulated with KDR-Ab and LY294002 as well as PD98059. But apoptosis inhibition effect induced by VEGF-C was not influenced by PD98059. Conclusions:In response to exogenous VEGF-C,the cells proliferation is increased as mediated by KDR,which involved the activation of PI3K or/and MAPK pathways through increasing the expression of Cyclin Dl to promote cells cycle phase S. Cell apoptosis is inhibited through cell signal transduction PI3K by increasing the expression of Bcl-2.关键词
VEGF-C/宫颈癌/增殖/凋亡/信号传导Key words
VEGF-C/Cervical cancer/Proliferation/Apoptosis/Cells signal transduction分类
医药卫生引用本文复制引用
杜雪,糜若然..VEGF-C对宫颈癌细胞增殖和凋亡信号传导通路的影响[J].现代妇产科进展,2011,20(11):877-880,885,5.
