现代妇产科进展2011，Vol.20Issue(12)：965-968,4.
溶血磷脂酸抑制顺铂诱导卵巢癌细胞凋亡机制的研究
Mechanisms involved in lysophosphatidic acid inhibited apoptosis induced by cisplatin in ovarian carcinoma cells
摘要
Abstract
Objective; To investigate the mechanisms of lysophosphatidic acid inhibited apoptosis induced by cisplatin in ovarian carcinoma cells. Methods; Human ovarian cancer cell line(SKOV3) was cultured, and MAPK inhibitor(PD98059) was used. The proliferation of SKOV3 cells stimulated by DDP,DDP+LPA and DDP+LPA+PD98059 was assessed by methyl thiazolyl tetrazolium(MTT). Cell apoptosis was detected by Hoechst33258. The expression of COX-2 in SKOV3 cells was assessed by RT-PCR. The apoptosis and cell cycle of SK0V3 cells were detected by flow cytometry(FCM). Results ;LPA could antagonize the inhibitory effect of DDP on the proliferation of SK0V3 cells,and increase the proportion of cells in the S phase of the cell cycle. However, PD98059 could significantly weaken these effect and increase apoptotic body. RT-PCR showed that LPA could increase the expression of COX-2 in SKOV3 cells (P< 0.05), while PD98059 could decrease it(P<0.05). Conclusion: LPA may inhibit the apoptosis induced by DDP through MAPK signaling pathway.关键词
溶血磷脂酸/卵巢肿瘤/MAPK信号传导通路/细胞凋亡Key words
Lysophosphatidic acid/ Ovarian neoplasms/ MAPK signaling pathway/ Ap-optosis分类
医药卫生引用本文复制引用
丁萍萍,李强..溶血磷脂酸抑制顺铂诱导卵巢癌细胞凋亡机制的研究[J].现代妇产科进展,2011,20(12):965-968,4.基金项目
山东省优秀中青年科学家科研奖励基金(No:03BS034) （No:03BS034）
