现代妇产科进展2012，Vol.21Issue(1)：12-16,5.
乙二醛酶Ⅰ介导孕激素对子宫内膜癌细胞增殖和凋亡活性的调控
Progestin-induced proliferative and apoptotic activity in endometrial cancer cell mediated by Glyoxalase Ⅰ
摘要
Abstract
Objective: To investigate whether MPA regulates the expession of Glo I through the progesterone receptor, following regulates endometrial cancer cell proliferation and apoptotic activity. Methods; Westen blot was used to detect the expression of Glo I , Caspase3, Cyclin Dl in protein levels after treatment with the progesterone; After the treatment of PR specific inhibitor, Westen blot was used to detect the expression of Glo I ,Caspase3 , Cyclin D;siR-NA was used to konck down the expression of Glo I gene and MTT assay was used to determine cellular growth,TUNEL was used to determine cellular apoptosis. Results: (1 )The expression of Glo I , Cyclin Dl could be down-regulated by MPA in Ishikawa cells and the expression of Caspase3 could be up-regulated by MPA in dose-dependent (P<0.05). (2)The expression of Glo I , Cyclin Dl was up-regulated and the Caspase3 was down-regulated by MPA±RU-486 compared with control group (P<0. 05). 3. Inhibition of Glo I by RNA interference could reduce cell proliferation and increase cell apoptosis (P<0. 05). Conclusion: MPA can regulatecell proliferation and apoptosis activity by Glo I gene through the progesterone receptor.关键词
子宫内膜癌/甲羟孕酮/孕激素受体/乙二醛酶Ⅰ/细胞增殖和凋亡Key words
Endometrial neoplasms/MPA/PR/Glo I /Cell proliferation and apoptosis分类
医药卫生引用本文复制引用
王倩倩,张箴波,丰有吉,廖虹,魏桠楠,吴凌,赖允丽,孙姗姗,赵贵旭,顾红琴,祝亚平..乙二醛酶Ⅰ介导孕激素对子宫内膜癌细胞增殖和凋亡活性的调控[J].现代妇产科进展,2012,21(1):12-16,5.基金项目
国家自然科学基金(No:81001155、81020108027) （No:81001155、81020108027）
上海市卫生局面上项目(No:2009028) （No:2009028）
