中国组织工程研究2012，Vol.16Issue(1)：39-42,4.DOI:10.3969/j.issn.1673-8225.2012.01.008
CD271磁珠体外诱导人胚胎干细胞分化为成骨细胞的可行性
Osteoblasts derived from human embryonic stem cells by CD271 magnetic beads in vitro
摘要
Abstract
BACKGROUND:Studies on osteoblasts edrived from human embryonic stem cells (hESCs)had made remarkable results,but the induced methods can produce a great number of effective osteoblasts edrived from hESCs without other miscellaneous cells is not established.OBJECTIVE:To explore the feasibility of osteoblasts derived form hESCs in vitro by using CD271 magnetic beads sorting system.METHODS:CD271 positive cells ewre obtained from hESCs lines SYSU-2 cells through embryoid bodies formation at 6 days and attachment differentiation at 14 days by using CD271 magnetic beads sorting technology,Cell surface antigen expression,karyotype analysis and potential of differentiation were detected on CD271 positive cells.RESULTS AND CONCLUTION:CD271 positive cells exhibited regular spindle shape,which was similar to morphology of bone marrow-derived mesenchymal stem cells(BMSCs),and could be cultured in vitro about 14 generation remaining stable laryotype.At the same time,these cells expressed similar cell surface antigen marde (CD45 negative;CD73,CD105 and CD166positive)with BMSCs.But differentiation potential identification showed that CD271 positive cells were solely induced osteoblasts formation.It has tremendous significance to study for the bone development mechanism and supply with enough,safe and efficient seed cells for bone tissue engineerig.关键词
磁珠分选/人胚胎干细胞/成骨细胞/CD271/骨组织工程分类
医药卫生引用本文复制引用
江虹虹,张金丽,李付贵,王涛..CD271磁珠体外诱导人胚胎干细胞分化为成骨细胞的可行性[J].中国组织工程研究,2012,16(1):39-42,4.基金项目
国家自然科学基金(81000362). （81000362）
