中国组织工程研究2012，Vol.16Issue(1)：61-64,4.DOI:10.3969/j.issn.1673-8225.2012.01.013
长期培养对基质细胞衍生因子1/CXCR4介导间充质干细胞迁移的影响
Stromal cell-derived factor-1/CXCR4 effects on ex vivo expanded bone marrow mesenchymal stem cells migration
摘要
Abstract
BACKGROUND: Exogenous human mesenchymal stem cells (MSCs) possess an ability to selectively migrate to the site of OBJECT IVE: To examine the expression of the CXCR4 receptor in MSCs after long-term culture ex vivo. and to investigate the effects of stromal cell derived factor-1 (SDF-1) at various concentrations on MSCs migration.METHODS: MSCs were cultured ex vivo to passage 10. and then cell senescence was determined by β-galactosidas estaining.The expression of C XC R 4 receptor in MSCs was examined byRT-PCR and immunofluor es cence staining. Transwell system wasused to detect in vitro migration capacity of MSCs towards SDF-1.RESULTS AND CONCLUSION: Proliferation gradually decreased in all samples in the course of Iong-term culture ex vivo. andbecame restricted to senescent stages at passage 6 or 7. The expression of CXC R4 receptor was gradually lost with cultureexpansion.SDF-1 induced the migration of MSCS in a dose- dependent manner.关键词
趋化作用/骨髓间充质干细胞/细胞衰老/基质细胞衍生因子1/CXCR4受体分类
医药卫生引用本文复制引用
王瑾,黄梁浒,王庆华,施小华,董会月,谭建明..长期培养对基质细胞衍生因子1/CXCR4介导间充质干细胞迁移的影响[J].中国组织工程研究,2012,16(1):61-64,4.基金项目
福建省科技计划重大项目(2009Y4001)和福建省科技创新平台建设计划(2010Y2006). （2009Y4001）
