中国组织工程研究2012，Vol.16Issue(1)：103-106,4.DOI:10.3969/j.issn.1673-8225.2012.01.022
不同质量浓度肉苁蓉总苷诱导大鼠骨髓间充质干细胞向神经细胞样细胞的定向分化
In vitro directional differentiation of bone marrow mesenchymal stem cells into nerve cells induced by cistanche total glycosides
董亚莉 1李剑锋1
作者信息
- 1. 内蒙古医学院第二附属医院康复科,内蒙古自治区呼和浩特市,010030
- 折叠
摘要
Abstract
BACKGROUND: Cistanche and its chemical com position play a neuroprotective role, and can promote recovery of neurological OBJECTIVE: To analyze the optimal dose of cistanche total glycosides for in vitro induction of bone marrow mesenchymalstem METHODS: Bone marrow mesen chymal stem cells of Wistar rats were isolated and then cultured, β-mercaptoethanol and glycosides ides groups of 100, 200, 300 μg/L for observation.RESULT SAND CONCLUSION: After 7 days of induction, the number of Irving cells increased obviously in other groups, compared with the blank control group (P < 0.05); at the 3rd day of induction. Nestin, NSE-posrtive expression rate was significantly higher in cistanche total glycosides groups, compared with the blank control group (P < 0.05). 100. 200 μ g/Lmesenchymal stem cells. but the effect was less than 100. 200 μg/L(P< 0.05).关键词
肉苁蓉总苷/骨髓间充质干细胞/定向分化/神经细胞样细胞/神经系统疾病分类
医药卫生引用本文复制引用
董亚莉,李剑锋..不同质量浓度肉苁蓉总苷诱导大鼠骨髓间充质干细胞向神经细胞样细胞的定向分化[J].中国组织工程研究,2012,16(1):103-106,4.
